TotiCyte\u2122 – the next generation of cord blood processing technology<\/h2>\n
The most important factors in determining a successful stem cell transplant are human leukocyte antigen (HLA) match and number of living (viable) cells available for treatment.
\nHLA is the finger print equivalent to see if the donor matches the patient. If they do not, then game over. However, if there is a good enough match, then 25 million total nucleated cells (TNC) per kg of bodyweight are required to give the patient the best chance of a successful stem cell transplant.
\nMany public cord blood banks apply a very high TNC threshold before they will store a cord blood unit for possible transplant use. Thresholds between 1.4 and 2.5 billion TNC increase the probability that the samples they process will be therapeutically useful.<\/p>\n
Volume reduction technologies<\/h3>\n
The leading methods of processng cord blood for storage employ a volume reduction step that aims to remove some of the non-useful red blood cell fraction and the plasma. For example, a starting volume of 100ml will be reduced to 25ml for final storage. Cells4Life has developed TotiCyte\u2122, a processing method which selectively removes the red blood cells in cord blood and yields:<\/p>\n TotiCyte\u2122 is a mixture of compounds already commonly used in blood product processing. When added to cord blood, gravity causes the red cells to selectively sediment within 30 minutes and the white cells remain in solution. These white cells can be easily separated from the sedimented red cells, and the important white cells can then be concentrated by gentle centrifugation into a small volume containing less than 0.5% of the original red cell content. Addition of a small amount of DMSO for cryogenic storage and controlled freezing using standard procedures then completes this simple process.<\/p>\n One of the most exciting features of TotiCyte\u2122 is improved post-thaw recovery. When cord blood stem cells are cryopreserved and then thawed for therapy, large cell losses occur during thawing. With TotiCyte\u2122, the viable cell recovery post-thaw is markedly increased. Approximately 76% of the CD34+ remains viable, whereas post-thaw viability with the current leading separation methodology can be as low as 32% in some instances (4)<\/sup>.<\/p>\n TotiCyte\u2122 allows almost the entire white cell fraction to be isolated and\/or concentrated, with only modest, if any, loss of any cell type thus far examined. Other processing methods frequently result in the loss of the megakaryocyte-like (CD45+CD61+) cells and\/or MNC-like (CD45+CD34+) cells.<\/p>\n TotiCyte\u2122 will positively impact the value chain of public cord blood banks in several ways:<\/p>\n In summary, our results show that TotiCyte\u2122 can deliver a larger number of viable cells for therapeutic use and more stem cell types than any other volume reduction technology. TotiCyte\u2122 – the next generation of cord blood processing technology The most important factors in determining a successful stem cell transplant are human leukocyte antigen (HLA) match and number of living (viable) cells available for treatment. HLA is the finger print equivalent to see if the donor matches the patient. If they do not, then […]<\/p>\n","protected":false},"author":1,"featured_media":3268,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_et_pb_use_builder":"","_et_pb_old_content":"","_et_gb_content_width":"","_themeisle_gutenberg_block_has_review":false,"footnotes":""},"categories":[1],"tags":[],"class_list":["post-1332","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-other"],"_links":{"self":[{"href":"https:\/\/cells4life.com\/us\/wp-json\/wp\/v2\/posts\/1332","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/cells4life.com\/us\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/cells4life.com\/us\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/cells4life.com\/us\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/cells4life.com\/us\/wp-json\/wp\/v2\/comments?post=1332"}],"version-history":[{"count":3,"href":"https:\/\/cells4life.com\/us\/wp-json\/wp\/v2\/posts\/1332\/revisions"}],"predecessor-version":[{"id":38754,"href":"https:\/\/cells4life.com\/us\/wp-json\/wp\/v2\/posts\/1332\/revisions\/38754"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/cells4life.com\/us\/wp-json\/wp\/v2\/media\/3268"}],"wp:attachment":[{"href":"https:\/\/cells4life.com\/us\/wp-json\/wp\/v2\/media?parent=1332"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/cells4life.com\/us\/wp-json\/wp\/v2\/categories?post=1332"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/cells4life.com\/us\/wp-json\/wp\/v2\/tags?post=1332"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
\nHowever, all volume reduction technologies result in stem cell losses approaching up to 40% (1)<\/sup>, and in some instances, the almost total loss of potentially important low abundance cells (2)<\/sup>. Significant levels of red blood cells remain, which can be up to 30% of the starting concentration (3)<\/sup>.<\/p>\n![\"TotiCyte\"](\"https:\/\/cells4life.com\/us\/wp-content\/uploads\/toticyte_table_RBC.gif\")
<\/p>\nTotiCyte\u2122 – a new paradigm in cell recovery<\/h3>\n
![\"Bullet\"](\"https:\/\/cells4life.com\/us\/wp-content\/uploads\/drop.gif\")
over 95% recovery of the white cell fraction
\n99.5% removal of the red cells
\nobviates the need for washing to remove DMSO and red cells, and
\nexceptionally high post thaw viable cell recovery<\/p>\nPost-thaw recovery<\/h3>\n
Entire white cell fraction<\/h3>\n
Impact for the public banking model<\/h3>\n
Cord blood processing with TotiCyte\u2122 will be cheaper than current methods.
\n\u0007Fewer donations will be wasted because the higher cell yield enables many more collections to meet the threshold for storage.
\nMore high TNC cord blood units in storage means more units that are desirable to be purchased for transplant.
\nBuilding the inventory of high TNC cord blood units also means that heavier patients can be transplanted, moving the therapeutic use segment from primarily children to adults.
\n\u0007TotiCyte\u2122\u2019s high TNC yield should enable only one cord blood unit to be used for larger adults, obviating the need for very expensive double cord blood transplants.<\/p>\n
\nThe public banking business model suffers from high wastage of donations and under-distribution of stored cord blood. TotiCyte\u2122 will increase public banking profitability both by reducing the number of donations that are discarded and by increasing the quality \/ marketability of the stored cord blood units.<\/p>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"